rat anti mouse pmn Search Results


rabbit anti pmn accurate antibodies antibodies  (Vector Laboratories)
96
Vector Laboratories rabbit anti pmn accurate antibodies antibodies
Rabbit Anti Pmn Accurate Antibodies Antibodies, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti pmn accurate antibodies antibodies/product/Vector Laboratories
Average 96 stars, based on 1 article reviews
rabbit anti pmn accurate antibodies antibodies - by Bioz Stars, 2026-05
96/100 stars
  Buy from Supplier
pmn  (Bio-Rad)
96
Bio-Rad pmn
( A ) Representative images of immunofluorescent staining of spinal cord with CD68 (green) and IRF8 (red) in WT mice at 4 and 7 dpi. The asterisks indicate the lesion epicenter. ( B ) CD68 (green) and IRF8 (red) double-positive macrophages in the perilesional areas. The nuclei were counterstained with Hoechst 33258 dye (blue). Selective localization of IRF8 in the nuclei of macrophages was observed at 7 dpi (bottom). All IRF8-expressing cells were CD68 + . ( C ) Fluorescence ratio (Fl. ratio) of nuclear IRF8 to cytosolic IRF8 ( n = 30 cells per group). ( D to H ) Double immunostaining of IRF8 with <t>PMN,</t> CD3, GFAP, NeuN, <t>and</t> <t>GST-π.</t> ( I ) The IRF8 expression examined by reverse transcription PCR (RT-PCR) in selectively isolated macrophages, neutrophils, reactive astrocytes, and neurons. Among these cells, the IRF8 expression was observed only in macrophages. The data shown in (I) are representative of three samples from three mice/each cell fraction. Images shown in (A), (B), and (D) to (H) are representative of eight sections per four mice. The images were obtained from two independent experiments. Scale bars, 500 μm (A), 20 μm (E and G), and 10 μm (B, D, F, and H). ** P < 0.005, Wilcoxon rank-sum test. The data are presented as means ± SEM. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
Pmn, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pmn/product/Bio-Rad
Average 96 stars, based on 1 article reviews
pmn - by Bioz Stars, 2026-05
96/100 stars
  Buy from Supplier
rabbit anti pmn antibody ab  (Cedarlane)
93
Cedarlane rabbit anti pmn antibody ab
( A ) Representative images of immunofluorescent staining of spinal cord with CD68 (green) and IRF8 (red) in WT mice at 4 and 7 dpi. The asterisks indicate the lesion epicenter. ( B ) CD68 (green) and IRF8 (red) double-positive macrophages in the perilesional areas. The nuclei were counterstained with Hoechst 33258 dye (blue). Selective localization of IRF8 in the nuclei of macrophages was observed at 7 dpi (bottom). All IRF8-expressing cells were CD68 + . ( C ) Fluorescence ratio (Fl. ratio) of nuclear IRF8 to cytosolic IRF8 ( n = 30 cells per group). ( D to H ) Double immunostaining of IRF8 with <t>PMN,</t> CD3, GFAP, NeuN, <t>and</t> <t>GST-π.</t> ( I ) The IRF8 expression examined by reverse transcription PCR (RT-PCR) in selectively isolated macrophages, neutrophils, reactive astrocytes, and neurons. Among these cells, the IRF8 expression was observed only in macrophages. The data shown in (I) are representative of three samples from three mice/each cell fraction. Images shown in (A), (B), and (D) to (H) are representative of eight sections per four mice. The images were obtained from two independent experiments. Scale bars, 500 μm (A), 20 μm (E and G), and 10 μm (B, D, F, and H). ** P < 0.005, Wilcoxon rank-sum test. The data are presented as means ± SEM. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
Rabbit Anti Pmn Antibody Ab, supplied by Cedarlane, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti pmn antibody ab/product/Cedarlane
Average 93 stars, based on 1 article reviews
rabbit anti pmn antibody ab - by Bioz Stars, 2026-05
93/100 stars
  Buy from Supplier
mouse anti pmn fcriii cd 16 mab  (Bio-Rad)
94
Bio-Rad mouse anti pmn fcriii cd 16 mab
( A ) Representative images of immunofluorescent staining of spinal cord with CD68 (green) and IRF8 (red) in WT mice at 4 and 7 dpi. The asterisks indicate the lesion epicenter. ( B ) CD68 (green) and IRF8 (red) double-positive macrophages in the perilesional areas. The nuclei were counterstained with Hoechst 33258 dye (blue). Selective localization of IRF8 in the nuclei of macrophages was observed at 7 dpi (bottom). All IRF8-expressing cells were CD68 + . ( C ) Fluorescence ratio (Fl. ratio) of nuclear IRF8 to cytosolic IRF8 ( n = 30 cells per group). ( D to H ) Double immunostaining of IRF8 with <t>PMN,</t> CD3, GFAP, NeuN, <t>and</t> <t>GST-π.</t> ( I ) The IRF8 expression examined by reverse transcription PCR (RT-PCR) in selectively isolated macrophages, neutrophils, reactive astrocytes, and neurons. Among these cells, the IRF8 expression was observed only in macrophages. The data shown in (I) are representative of three samples from three mice/each cell fraction. Images shown in (A), (B), and (D) to (H) are representative of eight sections per four mice. The images were obtained from two independent experiments. Scale bars, 500 μm (A), 20 μm (E and G), and 10 μm (B, D, F, and H). ** P < 0.005, Wilcoxon rank-sum test. The data are presented as means ± SEM. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
Mouse Anti Pmn Fcriii Cd 16 Mab, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti pmn fcriii cd 16 mab/product/Bio-Rad
Average 94 stars, based on 1 article reviews
mouse anti pmn fcriii cd 16 mab - by Bioz Stars, 2026-05
94/100 stars
  Buy from Supplier
mouse ly6g anti pmn  (Bio-Rad)
93
Bio-Rad mouse ly6g anti pmn
( A ) Representative images of immunofluorescent staining of spinal cord with CD68 (green) and IRF8 (red) in WT mice at 4 and 7 dpi. The asterisks indicate the lesion epicenter. ( B ) CD68 (green) and IRF8 (red) double-positive macrophages in the perilesional areas. The nuclei were counterstained with Hoechst 33258 dye (blue). Selective localization of IRF8 in the nuclei of macrophages was observed at 7 dpi (bottom). All IRF8-expressing cells were CD68 + . ( C ) Fluorescence ratio (Fl. ratio) of nuclear IRF8 to cytosolic IRF8 ( n = 30 cells per group). ( D to H ) Double immunostaining of IRF8 with <t>PMN,</t> CD3, GFAP, NeuN, <t>and</t> <t>GST-π.</t> ( I ) The IRF8 expression examined by reverse transcription PCR (RT-PCR) in selectively isolated macrophages, neutrophils, reactive astrocytes, and neurons. Among these cells, the IRF8 expression was observed only in macrophages. The data shown in (I) are representative of three samples from three mice/each cell fraction. Images shown in (A), (B), and (D) to (H) are representative of eight sections per four mice. The images were obtained from two independent experiments. Scale bars, 500 μm (A), 20 μm (E and G), and 10 μm (B, D, F, and H). ** P < 0.005, Wilcoxon rank-sum test. The data are presented as means ± SEM. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
Mouse Ly6g Anti Pmn, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse ly6g anti pmn/product/Bio-Rad
Average 93 stars, based on 1 article reviews
mouse ly6g anti pmn - by Bioz Stars, 2026-05
93/100 stars
  Buy from Supplier
polyclonal rabbit anti rat pmn antibody  (Cedarlane)
93
Cedarlane polyclonal rabbit anti rat pmn antibody
( A ) Representative images of immunofluorescent staining of spinal cord with CD68 (green) and IRF8 (red) in WT mice at 4 and 7 dpi. The asterisks indicate the lesion epicenter. ( B ) CD68 (green) and IRF8 (red) double-positive macrophages in the perilesional areas. The nuclei were counterstained with Hoechst 33258 dye (blue). Selective localization of IRF8 in the nuclei of macrophages was observed at 7 dpi (bottom). All IRF8-expressing cells were CD68 + . ( C ) Fluorescence ratio (Fl. ratio) of nuclear IRF8 to cytosolic IRF8 ( n = 30 cells per group). ( D to H ) Double immunostaining of IRF8 with <t>PMN,</t> CD3, GFAP, NeuN, <t>and</t> <t>GST-π.</t> ( I ) The IRF8 expression examined by reverse transcription PCR (RT-PCR) in selectively isolated macrophages, neutrophils, reactive astrocytes, and neurons. Among these cells, the IRF8 expression was observed only in macrophages. The data shown in (I) are representative of three samples from three mice/each cell fraction. Images shown in (A), (B), and (D) to (H) are representative of eight sections per four mice. The images were obtained from two independent experiments. Scale bars, 500 μm (A), 20 μm (E and G), and 10 μm (B, D, F, and H). ** P < 0.005, Wilcoxon rank-sum test. The data are presented as means ± SEM. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
Polyclonal Rabbit Anti Rat Pmn Antibody, supplied by Cedarlane, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polyclonal rabbit anti rat pmn antibody/product/Cedarlane
Average 93 stars, based on 1 article reviews
polyclonal rabbit anti rat pmn antibody - by Bioz Stars, 2026-05
93/100 stars
  Buy from Supplier
rat anti mouse pmn  (Bio-Rad)
96
Bio-Rad rat anti mouse pmn
( A ) Representative images of immunofluorescent staining of spinal cord with CD68 (green) and IRF8 (red) in WT mice at 4 and 7 dpi. The asterisks indicate the lesion epicenter. ( B ) CD68 (green) and IRF8 (red) double-positive macrophages in the perilesional areas. The nuclei were counterstained with Hoechst 33258 dye (blue). Selective localization of IRF8 in the nuclei of macrophages was observed at 7 dpi (bottom). All IRF8-expressing cells were CD68 + . ( C ) Fluorescence ratio (Fl. ratio) of nuclear IRF8 to cytosolic IRF8 ( n = 30 cells per group). ( D to H ) Double immunostaining of IRF8 with <t>PMN,</t> CD3, GFAP, NeuN, <t>and</t> <t>GST-π.</t> ( I ) The IRF8 expression examined by reverse transcription PCR (RT-PCR) in selectively isolated macrophages, neutrophils, reactive astrocytes, and neurons. Among these cells, the IRF8 expression was observed only in macrophages. The data shown in (I) are representative of three samples from three mice/each cell fraction. Images shown in (A), (B), and (D) to (H) are representative of eight sections per four mice. The images were obtained from two independent experiments. Scale bars, 500 μm (A), 20 μm (E and G), and 10 μm (B, D, F, and H). ** P < 0.005, Wilcoxon rank-sum test. The data are presented as means ± SEM. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
Rat Anti Mouse Pmn, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rat anti mouse pmn/product/Bio-Rad
Average 96 stars, based on 1 article reviews
rat anti mouse pmn - by Bioz Stars, 2026-05
96/100 stars
  Buy from Supplier
rat anti-mouse-c3  (Hycultec Inc)
90
Hycultec Inc rat anti-mouse-c3
( A ) Representative images of immunofluorescent staining of spinal cord with CD68 (green) and IRF8 (red) in WT mice at 4 and 7 dpi. The asterisks indicate the lesion epicenter. ( B ) CD68 (green) and IRF8 (red) double-positive macrophages in the perilesional areas. The nuclei were counterstained with Hoechst 33258 dye (blue). Selective localization of IRF8 in the nuclei of macrophages was observed at 7 dpi (bottom). All IRF8-expressing cells were CD68 + . ( C ) Fluorescence ratio (Fl. ratio) of nuclear IRF8 to cytosolic IRF8 ( n = 30 cells per group). ( D to H ) Double immunostaining of IRF8 with <t>PMN,</t> CD3, GFAP, NeuN, <t>and</t> <t>GST-π.</t> ( I ) The IRF8 expression examined by reverse transcription PCR (RT-PCR) in selectively isolated macrophages, neutrophils, reactive astrocytes, and neurons. Among these cells, the IRF8 expression was observed only in macrophages. The data shown in (I) are representative of three samples from three mice/each cell fraction. Images shown in (A), (B), and (D) to (H) are representative of eight sections per four mice. The images were obtained from two independent experiments. Scale bars, 500 μm (A), 20 μm (E and G), and 10 μm (B, D, F, and H). ** P < 0.005, Wilcoxon rank-sum test. The data are presented as means ± SEM. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
Rat Anti Mouse C3, supplied by Hycultec Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rat anti-mouse-c3/product/Hycultec Inc
Average 90 stars, based on 1 article reviews
rat anti-mouse-c3 - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier
rat anti-mouse pecam-1 primary antibody  (Becton Dickinson)
90
Becton Dickinson rat anti-mouse pecam-1 primary antibody
( A ) Representative images of immunofluorescent staining of spinal cord with CD68 (green) and IRF8 (red) in WT mice at 4 and 7 dpi. The asterisks indicate the lesion epicenter. ( B ) CD68 (green) and IRF8 (red) double-positive macrophages in the perilesional areas. The nuclei were counterstained with Hoechst 33258 dye (blue). Selective localization of IRF8 in the nuclei of macrophages was observed at 7 dpi (bottom). All IRF8-expressing cells were CD68 + . ( C ) Fluorescence ratio (Fl. ratio) of nuclear IRF8 to cytosolic IRF8 ( n = 30 cells per group). ( D to H ) Double immunostaining of IRF8 with <t>PMN,</t> CD3, GFAP, NeuN, <t>and</t> <t>GST-π.</t> ( I ) The IRF8 expression examined by reverse transcription PCR (RT-PCR) in selectively isolated macrophages, neutrophils, reactive astrocytes, and neurons. Among these cells, the IRF8 expression was observed only in macrophages. The data shown in (I) are representative of three samples from three mice/each cell fraction. Images shown in (A), (B), and (D) to (H) are representative of eight sections per four mice. The images were obtained from two independent experiments. Scale bars, 500 μm (A), 20 μm (E and G), and 10 μm (B, D, F, and H). ** P < 0.005, Wilcoxon rank-sum test. The data are presented as means ± SEM. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
Rat Anti Mouse Pecam 1 Primary Antibody, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rat anti-mouse pecam-1 primary antibody/product/Becton Dickinson
Average 90 stars, based on 1 article reviews
rat anti-mouse pecam-1 primary antibody - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier
rat anti-mouse cd3ε  (Becton Dickinson)
90
Becton Dickinson rat anti-mouse cd3ε
( A ) Representative images of immunofluorescent staining of spinal cord with CD68 (green) and IRF8 (red) in WT mice at 4 and 7 dpi. The asterisks indicate the lesion epicenter. ( B ) CD68 (green) and IRF8 (red) double-positive macrophages in the perilesional areas. The nuclei were counterstained with Hoechst 33258 dye (blue). Selective localization of IRF8 in the nuclei of macrophages was observed at 7 dpi (bottom). All IRF8-expressing cells were CD68 + . ( C ) Fluorescence ratio (Fl. ratio) of nuclear IRF8 to cytosolic IRF8 ( n = 30 cells per group). ( D to H ) Double immunostaining of IRF8 with <t>PMN,</t> CD3, GFAP, NeuN, <t>and</t> <t>GST-π.</t> ( I ) The IRF8 expression examined by reverse transcription PCR (RT-PCR) in selectively isolated macrophages, neutrophils, reactive astrocytes, and neurons. Among these cells, the IRF8 expression was observed only in macrophages. The data shown in (I) are representative of three samples from three mice/each cell fraction. Images shown in (A), (B), and (D) to (H) are representative of eight sections per four mice. The images were obtained from two independent experiments. Scale bars, 500 μm (A), 20 μm (E and G), and 10 μm (B, D, F, and H). ** P < 0.005, Wilcoxon rank-sum test. The data are presented as means ± SEM. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
Rat Anti Mouse Cd3ε, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rat anti-mouse cd3ε/product/Becton Dickinson
Average 90 stars, based on 1 article reviews
rat anti-mouse cd3ε - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier
rat anti-mouse tnf-a  (Centocor Inc)
90
Centocor Inc rat anti-mouse tnf-a
( A ) Representative images of immunofluorescent staining of spinal cord with CD68 (green) and IRF8 (red) in WT mice at 4 and 7 dpi. The asterisks indicate the lesion epicenter. ( B ) CD68 (green) and IRF8 (red) double-positive macrophages in the perilesional areas. The nuclei were counterstained with Hoechst 33258 dye (blue). Selective localization of IRF8 in the nuclei of macrophages was observed at 7 dpi (bottom). All IRF8-expressing cells were CD68 + . ( C ) Fluorescence ratio (Fl. ratio) of nuclear IRF8 to cytosolic IRF8 ( n = 30 cells per group). ( D to H ) Double immunostaining of IRF8 with <t>PMN,</t> CD3, GFAP, NeuN, <t>and</t> <t>GST-π.</t> ( I ) The IRF8 expression examined by reverse transcription PCR (RT-PCR) in selectively isolated macrophages, neutrophils, reactive astrocytes, and neurons. Among these cells, the IRF8 expression was observed only in macrophages. The data shown in (I) are representative of three samples from three mice/each cell fraction. Images shown in (A), (B), and (D) to (H) are representative of eight sections per four mice. The images were obtained from two independent experiments. Scale bars, 500 μm (A), 20 μm (E and G), and 10 μm (B, D, F, and H). ** P < 0.005, Wilcoxon rank-sum test. The data are presented as means ± SEM. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
Rat Anti Mouse Tnf A, supplied by Centocor Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rat anti-mouse tnf-a/product/Centocor Inc
Average 90 stars, based on 1 article reviews
rat anti-mouse tnf-a - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier
rat anti-mouse erhr3  (BMA Biomedicals)
90
BMA Biomedicals rat anti-mouse erhr3
( A ) Representative images of immunofluorescent staining of spinal cord with CD68 (green) and IRF8 (red) in WT mice at 4 and 7 dpi. The asterisks indicate the lesion epicenter. ( B ) CD68 (green) and IRF8 (red) double-positive macrophages in the perilesional areas. The nuclei were counterstained with Hoechst 33258 dye (blue). Selective localization of IRF8 in the nuclei of macrophages was observed at 7 dpi (bottom). All IRF8-expressing cells were CD68 + . ( C ) Fluorescence ratio (Fl. ratio) of nuclear IRF8 to cytosolic IRF8 ( n = 30 cells per group). ( D to H ) Double immunostaining of IRF8 with <t>PMN,</t> CD3, GFAP, NeuN, <t>and</t> <t>GST-π.</t> ( I ) The IRF8 expression examined by reverse transcription PCR (RT-PCR) in selectively isolated macrophages, neutrophils, reactive astrocytes, and neurons. Among these cells, the IRF8 expression was observed only in macrophages. The data shown in (I) are representative of three samples from three mice/each cell fraction. Images shown in (A), (B), and (D) to (H) are representative of eight sections per four mice. The images were obtained from two independent experiments. Scale bars, 500 μm (A), 20 μm (E and G), and 10 μm (B, D, F, and H). ** P < 0.005, Wilcoxon rank-sum test. The data are presented as means ± SEM. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
Rat Anti Mouse Erhr3, supplied by BMA Biomedicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rat anti-mouse erhr3/product/BMA Biomedicals
Average 90 stars, based on 1 article reviews
rat anti-mouse erhr3 - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier

Image Search Results


( A ) Representative images of immunofluorescent staining of spinal cord with CD68 (green) and IRF8 (red) in WT mice at 4 and 7 dpi. The asterisks indicate the lesion epicenter. ( B ) CD68 (green) and IRF8 (red) double-positive macrophages in the perilesional areas. The nuclei were counterstained with Hoechst 33258 dye (blue). Selective localization of IRF8 in the nuclei of macrophages was observed at 7 dpi (bottom). All IRF8-expressing cells were CD68 + . ( C ) Fluorescence ratio (Fl. ratio) of nuclear IRF8 to cytosolic IRF8 ( n = 30 cells per group). ( D to H ) Double immunostaining of IRF8 with PMN, CD3, GFAP, NeuN, and GST-π. ( I ) The IRF8 expression examined by reverse transcription PCR (RT-PCR) in selectively isolated macrophages, neutrophils, reactive astrocytes, and neurons. Among these cells, the IRF8 expression was observed only in macrophages. The data shown in (I) are representative of three samples from three mice/each cell fraction. Images shown in (A), (B), and (D) to (H) are representative of eight sections per four mice. The images were obtained from two independent experiments. Scale bars, 500 μm (A), 20 μm (E and G), and 10 μm (B, D, F, and H). ** P < 0.005, Wilcoxon rank-sum test. The data are presented as means ± SEM. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.

Journal: Science Advances

Article Title: Macrophage centripetal migration drives spontaneous healing process after spinal cord injury

doi: 10.1126/sciadv.aav5086

Figure Lengend Snippet: ( A ) Representative images of immunofluorescent staining of spinal cord with CD68 (green) and IRF8 (red) in WT mice at 4 and 7 dpi. The asterisks indicate the lesion epicenter. ( B ) CD68 (green) and IRF8 (red) double-positive macrophages in the perilesional areas. The nuclei were counterstained with Hoechst 33258 dye (blue). Selective localization of IRF8 in the nuclei of macrophages was observed at 7 dpi (bottom). All IRF8-expressing cells were CD68 + . ( C ) Fluorescence ratio (Fl. ratio) of nuclear IRF8 to cytosolic IRF8 ( n = 30 cells per group). ( D to H ) Double immunostaining of IRF8 with PMN, CD3, GFAP, NeuN, and GST-π. ( I ) The IRF8 expression examined by reverse transcription PCR (RT-PCR) in selectively isolated macrophages, neutrophils, reactive astrocytes, and neurons. Among these cells, the IRF8 expression was observed only in macrophages. The data shown in (I) are representative of three samples from three mice/each cell fraction. Images shown in (A), (B), and (D) to (H) are representative of eight sections per four mice. The images were obtained from two independent experiments. Scale bars, 500 μm (A), 20 μm (E and G), and 10 μm (B, D, F, and H). ** P < 0.005, Wilcoxon rank-sum test. The data are presented as means ± SEM. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.

Article Snippet: The following primary antibodies were used: CD68 (rat, MCA1957; 1:200; Serotec), GFAP (rabbit, Z0334; 1:200; Dako), IRF8 (goat, sc-6058; 1:200; Santa Cruz Biotechnology), GST-π (mouse, no. 610719; 1:200; BD Biosciences), NeuN (mouse, MAB377; 1:200; Chemicon), PMN (rat, MCA771GA; 1:200; Serotec), CD3 (145-2C11; 1:200; eBioscience), 5-HT (goat, no.20079; 1:200; ImmunoStar), platelet-derived growth factor receptor-β (PDGFRβ) (rat, no.136002; 1:200; BioLegend), and C5a (rabbit, BS-0324R; 1:200; Bioss).

Techniques: Staining, Expressing, Fluorescence, Double Immunostaining, Reverse Transcription, Reverse Transcription Polymerase Chain Reaction, Isolation